Device
Part:BBa_K2120305
Designed by: HongYu Yang Group: iGEM16_BIT-China (2016-10-13)
araC-pBAD+B0034+CI+B0015
<Description>
This part is composed of two elements:
(1) Arabinose induced promoter pBAD and its inhibitor araC BBa_K808000.This part contains the promoter as well as the coding sequence for the repressor araC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, araC changes its conformation. This causes the protein to diffuse from the DNA thereby inducing transcription.
(2) The pR promoter's inhibitor gene cI from bacteriophage lambda
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
[edit]
Categories
Parameters
None |